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isoelectric focusing (ief) strips  (Bio-Rad)


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    Structured Review

    Bio-Rad isoelectric focusing (ief) strips
    Isoelectric Focusing (Ief) Strips, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isoelectric+focusing+(ief)+strip/Isoelectric+Focusing+Standards/pmc03701575-88-6-14
    Average 90 stars, based on 1 article reviews
    isoelectric focusing (ief) strips - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Stripping Membranes:

    Article Title: Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE.
    Article Snippet: .. Isoelectric focusing was performed on a strip within pH 3–10 using a BioRad PROTEAN i12 IEF Cell (BioRad, Hercules, CA, USA) with gradient parameters: 250 V linear for 20 min, 4000 V linear for 2 h, rapid 4000–10 000 VHr, hold at 500 V and rehydration for 14 h. Next day the strip was dried on a paper towel and equilibrated in a tray with solution I (6 M Urea, 2% SDS, 0.375 M Tris–HCl pH 8.8, 20% glycerol, 2%, DTT) and solution II (6 M Urea, 2% SDS, 0.375 M Tris–HCl 12 Vol:. (1234567890) Scientific Reports | (2022) 12:7520 | https://doi.org/10.1038/s41598-022-11638-2 pH 8.8, 20% glycerol, iodoacetamide) for 10 min each. .. The second-dimension electrophoresis was carried on 8% polyacrylamide gel at 200 V for 42 min. Next the gel was rinsed 3 × for 10 min with miliQ water and stained with Bio-Safe Coomassie Stain (BioRad, Hercules, CA, USA) or transferred to the PVDF membrane at 150 V for 90 min.

    Article Title: Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis
    Article Snippet: .. The strips were focused at 50 μA/IPG strip for a total of 60 kVh at 20°C using the Protean IEF Cell (BioRad). ..

    Article Title: Comparative growth, peroxidase activity and 2-D proteome analyses of halophytic and xerophytic species of Haloxylon under NaCl stress
    Article Snippet: Differential NaCl tolerance efficiency of salt-tolerant Haloxylon recurvum and drought-tolerant Haloxylon salicornicum was investigated in the present study.. The analyses were based on growth, peroxidase activity and 2-D protein profiling of 0, 50, 100, 150, 200, 250 mM NaCl treated cotyledonary nodes and shoots of both the species.. NaCl promoted the growth of cotyledonary nodes of H. recurvum up to 100 mM but found to be inhibitory for H. salicornicum.

    Article Title: Structural and functional characterization of colonization factors AIBI-CS6 and AIIBII-CS6 of enterotoxigenic Escherichia coli.
    Article Snippet: Over time, the structure and function of the broadly dispersed colonization factor (CF) CS6 of enterotoxigenic Escherichia coli (ETEC) have become more significant.. CS6 is composed of tightly-associated subunits, CssA and CssB which due to presence of natural point mutation gave rise to CS6 subtypes.. In contrast to the other ETEC CFs, CS6 is an afimbrial, spherical-shaped oligomers of (CssA-CssB)n complex where ‘n’ is concentration dependent.

    Electrofocusing:

    Article Title: Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE.
    Article Snippet: .. Isoelectric focusing was performed on a strip within pH 3–10 using a BioRad PROTEAN i12 IEF Cell (BioRad, Hercules, CA, USA) with gradient parameters: 250 V linear for 20 min, 4000 V linear for 2 h, rapid 4000–10 000 VHr, hold at 500 V and rehydration for 14 h. Next day the strip was dried on a paper towel and equilibrated in a tray with solution I (6 M Urea, 2% SDS, 0.375 M Tris–HCl pH 8.8, 20% glycerol, 2%, DTT) and solution II (6 M Urea, 2% SDS, 0.375 M Tris–HCl 12 Vol:. (1234567890) Scientific Reports | (2022) 12:7520 | https://doi.org/10.1038/s41598-022-11638-2 pH 8.8, 20% glycerol, iodoacetamide) for 10 min each. .. The second-dimension electrophoresis was carried on 8% polyacrylamide gel at 200 V for 42 min. Next the gel was rinsed 3 × for 10 min with miliQ water and stained with Bio-Safe Coomassie Stain (BioRad, Hercules, CA, USA) or transferred to the PVDF membrane at 150 V for 90 min.

    Article Title: Transcriptomic and proteomic analyses reveal the first occurrence of diverse toxin groups in Millepora alcicornis.
    Article Snippet: Millepora alcicornis is a reef-forming cnidarian widely distributed in the Mexican Caribbean.. Millepora species or “fire corals" inflict a painful stinging reaction in humans when touched.. Even though hundreds of organic and polypeptide toxins have been characterized from sea anemones and jellyfish, there are few reports regarding the diversity of toxins synthesized by fire corals.

    Article Title: Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis
    Article Snippet: .. The strips were focused at 50 μA/IPG strip for a total of 60 kVh at 20°C using the Protean IEF Cell (BioRad). ..

    Article Title: Comparative growth, peroxidase activity and 2-D proteome analyses of halophytic and xerophytic species of Haloxylon under NaCl stress
    Article Snippet: Differential NaCl tolerance efficiency of salt-tolerant Haloxylon recurvum and drought-tolerant Haloxylon salicornicum was investigated in the present study.. The analyses were based on growth, peroxidase activity and 2-D protein profiling of 0, 50, 100, 150, 200, 250 mM NaCl treated cotyledonary nodes and shoots of both the species.. NaCl promoted the growth of cotyledonary nodes of H. recurvum up to 100 mM but found to be inhibitory for H. salicornicum.

    Article Title: Structural and functional characterization of colonization factors AIBI-CS6 and AIIBII-CS6 of enterotoxigenic Escherichia coli.
    Article Snippet: Over time, the structure and function of the broadly dispersed colonization factor (CF) CS6 of enterotoxigenic Escherichia coli (ETEC) have become more significant.. CS6 is composed of tightly-associated subunits, CssA and CssB which due to presence of natural point mutation gave rise to CS6 subtypes.. In contrast to the other ETEC CFs, CS6 is an afimbrial, spherical-shaped oligomers of (CssA-CssB)n complex where ‘n’ is concentration dependent.

    other:

    Article Title: Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma.
    Article Snippet: In the first-dimensional (1DE) analysis, 125 μL of 150 μg protein was loaded onto the isoelectric focusing (IEF) strip (Non-linear, pH 3–10, 7 cm, Bio-Rad, Hercules, CA, USA).

    Article Title: Autoantibody against Tumor-Associated Antigens as Diagnostic Biomarkers in Hispanic Patients with Hepatocellular Carcinoma
    Article Snippet: In the first-dimensional (1DE) analysis, 125 μL of 150 μg protein was loaded onto the isoelectric focusing (IEF) strip (Non-linear, pH 3–10, 7 cm, Bio-Rad, Hercules, CA, USA).

    Article Title: Identification of Novel Amelogenin-Binding Proteins by Proteomics Analysis
    Article Snippet: The purified protein samples, diluted with 125 μL of rehydration buffer (Bio-Rad), were loaded onto an isoelectric focusing (IEF) strip (7 cm, pH 3–10; BioRad) in a PROTEAN IEF Cell (Bio-Rad).



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